I really can't stand Qiagen miniprep and maxiprep kits. They're for isolating DNA from cultures of bacteria cells, so you can end up with a tube full of clean, pure copies of a plasmid.
And these kits are designed to be foolproof. It's really about just adding a solution, shaking, adding another solution, shaking, adding another solution, spinning it in a centrifuge and voila, you're supposed to have a high concentration tube of plasmid. It's supposed to be foolproof.
So what does it say about me -- I can't get the damn things to work!
Argh.
Tomorrow is my third stab at the Miniprep kit. And I swear I've done it successfully before. I'm not an idiot!!
Ah, well, maybe the third time's the charm?
